Acanthocheilonema reconditum, dracunculoides (qPCR)

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Description

The Acanthocheilonema reconditum, dracunculoides (qPCR) product is used to detect sequences specific to the nematodes Acanthocheilonema reconditum and Acanthocheilonema dracunculoides in DNA preparations obtained from dog blood.

Product characteristics

Kit size: 100 reactions

Reaction: triplex (FAM: A. reconditum, HEX: internal control), Texas Red: A. dracunculoides

Internal control: exogenous/endogenous

Determination: qualitative/quantitative

Kit components:

  • Reaction mixture: contains DNA polymerase, probes and primers, and other qPCR reaction components;
  • Positive control;
  • Negative control;
  • PCR-grade water

Description of nematodes Acanthocheilonema

The nematodes Acanthocheilonema dracunculoides and Acanthocheilonema reconditum are parasites found in dogs worldwide. Often they occur alongside the more well-known nematodes Dirofilaria immitis and Dirofilaria repens. Although they are considered less pathogenic than heartworms, their presence in a dog’s body can lead to certain health and diagnostic problems.

A. dracunculoides is found in the peritoneal cavity and adipose tissue of its host. This parasite is found not only in dogs, but also in wild predators such as spotted hyenas, red foxes, and coyotes. It is found on three continents – Europe, Asia, and Africa. It is transmitted by flies (including Hippobosca longipennis) and dog ticks (Rhipicephalus sanguineus). Although it has long been considered non-pathogenic, there are reports suggesting that its presence may cause mild clinical symptoms in dogs.

Acanthocheilonema reconditum, on the other hand, most often inhabits the subcutaneous tissue – mainly of the back, but also the hind limbs, torso, and kidney area. It is commonly found in the Mediterranean basin, South America, Oceania, and South Africa, among other places. In some regions, it is even the most common filaria in dogs. For example, in Italy, the infection rate reaches 13.3%. Usually it does not cause any symptoms. However, the microfilariae of this nematode circulate in the blood, which can lead to misdiagnosis. They are confused with the microfilariae of D. immitis and D. repens, which cause serious diseases.

From the perspective of dog health, infections with nematodes of the genus Acanthocheilonema are relatively mild, but their detection can be difficult and lead to unnecessary treatment of more serious diseases. In addition, more and more attention is being paid to the potential threat that A. reconditum may also pose to humans. It is therefore important to carry out accurate diagnostics and consult the results with an experienced veterinarian.

Files to download

Safety Data Sheet

Rivbio product catalog