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The Alternaria (qPCR) product is used to detect sequences specific to fungi of the genus Alternaria in DNA preparations obtained from biological material collected from flax.
Kit size: 100 reactions
Reaction: duplex (FAM: Alternaria, HEX: internal control)
Internal control: exogenous/endogenous
Determination: qualitative/quantitative
Kit components:
Alternaria is a ubiquitous genus of fungi that includes saprobionts, endophytes, and pathogenic species associated with a wide range of infected plants. In addition, these fungi are a common allergen for humans. This genus includes over 360 recognized species, which are currently divided into 29 sections. These fungi are also well known as pathogens of crops (cereal grains).
Some species are clinically significant because they produce toxic secondary metabolites, some of which are potent mycotoxins associated with the development of cancer in mammals. In particular, A. alternata is gaining importance as a new human pathogen, especially in immunocompromised patients. In addition, Alternaria spores are one of the most common airborne allergens.
Alternaria fungi cause diseases in over 400 plant species. During colonization, these fungi can produce various toxins derived from secondary metabolism. To date, approximately 70 toxic metabolites have been described. The genus is one of the most common types of fungi found in grains in many regions of the world. The frequency of occurrence of these fungi in grainsis very high and can reach over 90%.
The most commonly reported species include A. alternata, A. tenuissima, A. arborescens, A. radicina, A. brassicae, A. brassicicola, and A. infectoria. They colonize a range of plants, including grains, oilseeds, tomatoes, cucumbers, cauliflowers, peppers, apples, melons, mandarins, oranges, lemons, and sunflower seeds. Colonization of ripening ears can lead to black, pointed kernels and have a direct impact on the color of wheat flour for baking bread.
