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The Anaplasma/Ehrlichia (qPCR) product is used to detect sequences specific to bacteria of the genera Anaplasma and Ehrlichia in DNA preparations obtained from ticks and material collected from animals.
Kit size: 100 markers
Reaction: duplex (FAM: Anaplasma/Ehrlichia, HEX: internal control)
Internal control: exogenous/endogenous
Determination: qualitative/quantitative
Kit components:
Bacteria of the genera Anaplasma and Ehrlichia are Gram-negative, obligate, intracellular pathogens belonging to the family Anaplasmataceae, transmitted by ticks of the genus Ixodes. They cause diseases known as anaplasmosis and ehrlichiosis in humans and animals, which can be severe and even life-threatening. The most common causative agents in humans are Anaplasma phagocytophilum, which causes human granulocytic anaplasmosis (HGA), and Ehrlichia chaffeensis, which causes human monocytic ehrlichiosis (HME). The symptoms of both diseases are similar and include high fever, headache, muscle pain, leukopenia, thrombocytopenia, and elevated liver enzymes. Symptoms usually appear within 5–10 days of the tick bite and can rapidly worsen.
In dogs, infections are mainly caused by Ehrlichia canis, E. ewingii, E. chaffeensis, Anaplasma phagocytophilum, and A. platys. The disease manifests itself in animals as fever, lethargy, loss of appetite, enlarged lymph nodes and spleen, hemorrhagic diathesis, and joint symptoms. The chronic form of ehrlichiosis in dogs is particularly dangerous, as it can lead to hematological disorders and multiple organ failure. Anaplasmosis caused by A. platys causes recurrent thrombocytopenia, which is often asymptomatic.
Although infections have also been reported in cats, clinical diseases caused by these bacteria are rare and poorly understood. Early and accurate molecular diagnostics allow for rapid detection of infection and initiation of treatment, which is crucial for preventing complications. Diagnostics based on the qPCR method is currently the most sensitive tool for detecting Anaplasma and Ehrlichia infections in both humans and animals.
