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The Anaplasma phagocytophilum (qPCR) product is used to detect sequences specific to the Anaplasma phagocytophilum bacterium in DNA preparations obtained from the blood of dogs and other animals, as well as in DNA preparations obtained from ticks.
Kit size: 100 reactions
Reaction: duplex (FAM: Anaplasma phagocytophilum, HEX: internal control)
Internal control: exogenous/endogenous
Determination: qualitative/quantitative
Kit components:
Anaplasma phagocytophilum is an obligate intracellular rickettsial pathogen transmitted by Ixodes ticks. This bacterium is a well-documented pathogen in both veterinary and human medicine. A. phagocytophilum causes tick-borne fever (TBF) in domestic ruminants and horses. In humans, infection with this pathogen causes human granulocytic anaplasmosis (HGA).
The main health problems associated with tick-borne fever in ruminants occur in young animals and individuals purchased from tick-free areas and placed for the first time in pastures infested with ticks. The most characteristic symptoms in domestic ruminants are high fever, anorexia, lethargy, and a sudden drop in milk production. Miscarriages in ewes and reduced fertility in rams have also been reported. Moreover, reduced weight gain has been observed in bulls and lambs infected with A. phagocytophilum.
In horses, clinical symptoms include fever, anorexia, depression, apathy, distal edema, reluctance to move, and petechiae. In dogs, tick-borne fever is characterized by fever, depression, lameness, and anorexia. The dominant symptoms in cats are anorexia, lethargy, hypersensitivity, conjunctivitis, muscle pain, joint pain, lameness, and lack of coordination.
Most infections with A. phagocytophilum in humans are asymptomatic or cause minimal clinical symptoms. More significant clinical symptoms range from mild, self-limiting febrile illness to fatal infections. Typically, symptomatic patients present with nonspecific flu-like symptoms including fever, headache, muscle aches, and malaise. In addition, thrombocytopenia, leukopenia, anemia, and increased serum aspartate and alanine aminotransferase activity have been reported.
