Description
The Blastocystis subtypes (qPCR) product is used to detect sequences specific to subtypes of protozoa of the genus Blastocystis in DNA preparations obtained from human feces.
Product characteristics
Kit size: 100 reactions
Reaction: duplex (FAM: Blastocystis subtypes, HEX: internal control)
Internal control: exogenous/endogenous
Determination: qualitative/quantitative
Kit components:
- Reaction mixture: contains DNA polymerase, probes and primers, and other qPCR reaction components;
- Positive control;
- Negative control;
- PCR-grade water;
- Internal control
Description of protozoa of the genus Blastocystis
Blastocystis is a genus of single-celled parasitic organisms that infect the gastrointestinal tract of humans and other vertebrates. It is the most common protozoan found in human stool samples and has a wide geographical distribution. The prevalence of Blastocystis in developing countries ranges from 30 to 50%. For most of the 20th century, the prevailing view was that these infections were harmless and asymptomatic. However, in the late 1980s, reports emerged linking Blastocystis with pathogenicity, mainly in travelers returning from underdeveloped countries.
Symptoms associated with blastocystosis include diarrhea, watery or loose stools, anal itching, abdominal pain, weight loss, and excess gas. Some studies link Blastocystis infections with acute or chronic digestive disorders. This parasite is transmitted via the fecal-oral route, through water and food. Due to its high prevalence in various animal species, the hypothesis of zoonotic transmission of this parasite between animals and humans has also been proposed.
Initially, strains of Blastocystis found in different hosts were classified into different species. For example, strains found in the human digestive tract were assigned to the species B. hominis. However, further research has shown that B. hominis does not exist as a separate entity, i.e., there is no single Blastocystis species that infects humans.
Therefore, in 2007, it was proposed to discontinue the use of the term B. hominis. Instead, it was proposed to divide the genus Blastocystis into individual subtypes based on the variability of the gene encoding the small ribosomal subunit (SSU). To date, 28 subtypes (ST1-ST28) have been described. The first nine subtypes and ST12 were isolated from the human gastrointestinal tract. In addition, isolated cases of subtypes ST10, ST13, ST14, and ST16 have been reported in humans. The most common subtypes in humans are ST3, ST1, ST2, and ST4.



