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The Bordetella bronchiseptica (qPCR) product is used to detect sequences characteristic of the Bordetella bronchiseptica bacterium in DNA preparations obtained from swabs taken from the upper respiratory tract of mammals.
Kit size: 100 reactions
Reaction: duplex (FAM: Bordetella bronchiseptica, HEX: internal control)
Internal control: exogenous/endogenous
Determination: qualitative/quantitative
Kit components:
Bordetella bronchiseptica is a small, Gram-negative, rod-shaped bacterium. It can cause infectious bronchitis in dogs and other animals, but rarely infects humans. It is closely related to B. pertussis, an obligate human pathogen that causes whooping cough (pertussis).
Bordetella bronchiseptica is the most common bacteriological cause of kennel cough or canine infectious respiratory disease (CIRD). CIRD is a highly contagious and multifactorial upper respiratory tract syndrome in dogs. Healthy dogs can be carriers of B. bronchiseptica. After the onset of clinical signs of CIRD caused by B. bronchiseptica dogs can spread the bacteria for up to three months. This makes it difficult to eliminate the bacteria from the environment. Clinical signs of CIRD include dry cough, accumulation of mucus in the trachea, vomiting, and pneumonia. Symptoms usually last 1-3 weeks, after which temporary immunity develops.
B. bronchiseptica is primarily an animal pathogen. In addition to CIRD in dogs, B. bronchiseptica causes sniffles in rabbits, atrophic rhinitis in pigs, and primary bronchitis and pneumonia in piglets. This microorganism can also cause chronic infections in humans, usually in immunocompromised individuals. Infection with B. bronchiseptica is not the only cause of CIRD. Other important causes include canine parainfluenza virus, canine respiratory coronavirus, and other viruses.
B. bronchiseptica infections are also widespread in the feline population, although they are often asymptomatic, as evidenced by high seroprevalence and bacterial isolation rates, even in healthy animals. The risk of infection increases with increasing numbers of cats in a single location and with poor hygiene conditions. The bacterium spreads primarily through contact with nasal and oral secretions, and its survival in the environment can be relatively long, allowing for indirect transmission. Contact with infected dogs is also a significant risk factor, as cross-species transmission has been confirmed.
The development of the disease in cats often depends on coexisting factors, such as environmental stress, crowding, or concurrent viral infections. Although B. bronchiseptica can cause disease on its own, its actual role as a sole pathogen in natural conditions is difficult to clearly assess. ptomsHowever, its ability to colonize the respiratory tract and produce toxins confirms its importance in the etiology of infections in cats.
