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The BVDV (qPCR) product is used to detect sequences specific to Bovine Viral Diarrhea Virus (BVDV) in RNA preparations obtained from cattle tissues.
Kit size: 100 reactions
Reaction: fourplex (FAM: BVDV-1, Texas Red: BVDV-2, Cy5: BVDV-3, HEX: internal control)
Internal control: exogenous/endogenous
Determination: qualitative/quantitatives
Kit components:
BVDV is a pestivirus belonging to the Flaviviridae family. The virus genome consists of a single positive-strand RNA. Bovine viral diarrhea/mucosal disease (BVD/MD) is one of the most important viral diseases and one of the most complex diseases affecting cattle. Due to the genetic diversity of the virus, BVDV is divided into two types: type 1 (BVDV-1) and type 2 (BVDV-2). Another pestivirus, referred to as HoBi-like pestivirus or BVDV-3, has recently been discovered. In Europe, this type of virus has been found in Italy. BVDV-3 has been linked to respiratory diseases and miscarriages.
Symptoms of BVDV infection may be subclinical, but also include abortion, congenital abnormalities, reduced fertility, persistent infection (PI) with gradual decline, and acute and fatal disease. The presence of antibodies, whether from passive transfer or vaccination, does not necessarily guarantee protection against various forms of the disease.
The acute form of the disease, caused by type 2 BVDV, occurs in individuals with reduced immunity. After an incubation period of 5–7 days, clinical signs include fever, anorexia, ocular and nasal discharge, mouth ulcers (including on the hard palate), diarrhea, and reduced milk production. The course of the disease may be shorter with hemorrhagic syndrome and death within 2 days. Clinical signs of infection in calves also include severe enteritis and pneumonia.
Infection with Bovine Viral Diarrhea Virus can occur during the fetal stage of life. In such cases, calves are usually healthy at birth, show resistance to the virus, and are chronically infected. Although they do not show any symptoms of the disease themselves, they are a source of infection for healthy individuals.
