Description
The Chlamydia/Chlamydophila (qPCR) product is used to detect sequences specific to bacteria of the genera Chlamydia and Chlamydophila in DNA preparations obtained from biological material collected from domestic and farm animals.
Product characteristics
Kit size: 100 reactions
Reaction: duplex (FAM: Chlamydia/Chlamydophila, HEX: internal control)
Internal control: exogenous/endogenous
Determination: qualitative/quantitative
Kit components:
- Reaction mixture: contains DNA polymerase, probes and primers, and other qPCR reaction components;
- Positive control;
- Negative control;
- PCR-grade water
Description of Chlamydia/Chlamydophila pathogens
The genera Chlamydia and Chlamydophila belong to the family Chlamydiaceae, which includes species of bacteria that are obligate intracellular parasites. Three species from this family are pathogenic to humans: Chlamydia trachomatis, Chlamydia psittaci, and Chlamydophila pneumoniae. The first of these (C. trachomatis) infects only humans, causing trachoma and reproductive tract infections. The other two species can also infect animals. Chlamydia suis is found in the digestive system of most pigs. It is a common cause of diarrhea in piglets before and after weaning. Chlamydophila pneumoniae infects not only humans but also horses and koalas. In humans, this pathogen mainly causes pneumonia.
Chlamydophila psittaci is mainly a pathogen of birds, causing psittacosis (parrot fever, ornithosis). However, this species can also infect cats, causing pneumonia, and pigs, causing bronchitis and interstitial pneumonia, arthritis, gastrointestinal tract inflammation, brain inflammation, miscarriage in females, and inflammation of the reproductive organs in boars. Chlamydophila abortus is the cause of enzootic abortion in sheep (ovine chlamydophilosis, enzootic ovine abortion), characterized by miscarriages, premature births, or the birth of weak lambs.
Chlamydophila felis causes respiratory tract inflammation and conjunctivitis in cats. In dogs infected with this pathogen, conjunctivitis, keratitis, encephalitis, and pneumonia occur. Chlamydophila pecorum is a pathogen of cattle. Most infections are asymptomatic. However, in young animals, infection can lead to pneumonia, arthritis, inflammation of the cornea and conjunctiva of the eye, and occasionally inflammation of the brain, spinal cord, and gastrointestinal tract. Other species that infect economically important animals include Chlamydia muridorum (infects hamsters and rodents) and Chlamydophila caviae (pathogen of guinea pigs).

