Chlamydia felis (qPCR)

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Description

The Chlamydia felis (qPCR) product is used to detect sequences specific to Chlamydia felis bacteria in DNA preparations obtained from swabs taken from the eyes/conjunctiva and upper respiratory tract of cats.

Product characteristics

Kit size: 100 reactions

Reaction: duplex (FAM: Chlamydia felis, HEX: internal control)

Internal control: exogenous/endogenous

Determination: qualitative/quantitative

Kit components:

  • Reaction mixture: contains DNA polymerase, probes and primers, and other qPCR reaction components;
  • Positive control;
  • Negative control;
  • PCR-grade water

Description of Chlamydia felis pathogen

Chlamydia felis is a small, Gram-negative bacterium that is an obligate intracellular parasite infecting cats. This bacterium was also previously referred to as Chlamydophila felis and Chlamydophila psittaci var. felis. C. felis is endemic among domestic cats worldwide, primarily causing conjunctivitis, rhinitis, and respiratory problems. Its presence has also been found in the stomach and reproductive tract of cats.

Chlamydia felis lives in mucous tissues. It cannot survive outside the host organism. In cats, the bacterium shows the greatest affinity for conjunctival cells. The bacterium is transmitted through direct contact between animals. The most common source of infection is secretions from the eyes of an infected cat. Infections with this bacterium are most common in environments with a high density of cats, particularly in cat breeding facilities. Its presence is found in 30% of cases of conjunctivitis. The most vulnerable to infection are kittens aged 5 weeks to several months, as well as immunocompromised cats, e.g. those suffering from FIV (feline immunodeficiency virus) or FeLV (feline leukemia virus).

Symptoms of C. felis infection usually appear within 2-5 days of infection. They include acute conjunctivitis with extreme mucosal hyperemia, eyelid spasm, and eye discomfort. The discharge from the eyes is initially watery, but becomes mucous or mucopurulent over time. A characteristic feature of chlamydiosis is conjunctival chemosis. Conjunctival desquamation usually stops within 60 days of infection. However, some cats may develop a persistent infection.

Files to download

Safety Data Sheet

Rivbio product catalog