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The Cryptosporidium spp. (qPCR) product is used to detect sequences specific to protozoa of the genus Cryptosporidium in DNA preparations obtained from human feces.
Kit size: 100 reactions
Reaction: duplex (FAM: Cryptosporidium spp., HEX:internal control)
Internal control: exogenous/endogenous
Determination: qualitative/quantitative
Kit components:
The genus Cryptosporidium (sometimes called crypto) consists of parasitic protozoa that can cause disease in humans and animals. Infections with Cryptosporidium protozoa cause cryptosporidiosis. In humans, this disease usually manifests itself as self-limiting diarrhea, but it can be severe, especially in immunocompromised individuals. However, there have also been reports of persistent gastrointestinal symptoms such as diarrhea, abdominal pain, nausea, fatigue, and headache. A possible link between Cryptosporidium infection and the development of inflammatory bowel disease and microscopic intestinal inflammation has also been suggested.
To date, infections with 21 species of this genus have been reported in humans. Most human cases are caused by infection with C. parvum or C. hominis, which account for more than 95% of human infections worldwide. These two species differ in their host range and global distribution. C. parvum infection has a wider host range, including ruminants, primates, equids, and rodents. C. hominis, on the other hand, is mainly limited to humans and other primates and equids. Other species that cause infection in humans include C. mortiferum, C. meleagridis, C. cuniculus, C. ubiquitum, C. canis, C. felis, C. viatorum, and C. ditrichi.
Cryptosporidiosis is mainly transmitted via the fecal-oral route, i.e., by ingesting live oocysts of animal and/or human origin that are excreted in feces and contaminate food or water. Although transmission of the infectious pathogen through consumption of contaminated water is well documented, neither the natural reservoir nor the exact route of crypto infection are well known.
