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The Ehrlichia canis (qPCR) product is used to detect sequences characteristic of the Ehrlichia canis bacterium in DNA preparations obtained from the blood of dogs and other animals (cats, horses) and in DNA preparations obtained from ticks.
Kit size: 100 reactions
Reaction: duplex (FAM: Ehrlichia canis, HEX: internal control)
Internal control: exogenous/endogenous
Determination: qualitative/quantitative
Kit components:
Ehrlichia canis is an obligate intracellular bacterium that causes ehrlichiosis, a disease that most commonly affects dogs. This bacterium also infects other animal species (including cats and horses) as well as humans. This pathogen is present throughout the United States, South America, Asia, Africa, Europe, and Australia. E. canis is transmitted by a tick vector from the Ixodidae family or the Rhipicephalus sanguineus species. Human infection can also occur through medical procedures. These include blood transfusions, organ transplants, or bone marrow transplants.
The symptoms of canine monocytic ehrlichiosis (CME) may vary depending on the form of the disease: acute, subclinical, or chronic with nonspecific clinical signs. The acute form lasts for 2–4 weeks and may include clinical signs such as fever, lethargy, depression, splenomegaly, anorexia, lymphadenopathy, weight loss, thrombocytopenia, anemia, hypergammaglobulinemia, pancytopenia, hemorrhages, epistaxis, and/or vomiting. Dogs with weakened immune systems are particularly vulnerable to this form.
In subacute or chronic cases of CME, the microorganism may be present in the circulatory system at undetectable levels or sequestered in tissues. This period ranges from several months to several years, and typical symptoms include depression and weight loss. The chronic form can progress to a severe form, with high fever, persistent mucosal bleeding, severe anemia, leukopenia, and thrombocytopenia.
In addition to E. canis, dogs can be infected by two other species of bacteria from the Ehrlichia genus: E. ewingii and E. platys. The fist species, along with Anaplasma phagocytophilum, causes a disease known as granulocytic ehrlichiosis. E. platys infection, on the other hand, leads to the development of thrombocytic ehrlichiosis.
