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The Francisella tularensis (qPCR) product is used to detect sequences specific to the bacterium Francisella tularensis in DNA preparations obtained from the blood of dogs and other animals, as well as in DNA preparations obtained from ticks.
Kit size: 100 reactions
Reaction: duplex (FAM: Francisella tularensis, HEX: internal control)
Internal control: exogenous/endogenous
Determination: qualitative/quantitative
Kit components:
Francisella tularensis, the etiological agent of tularemia, is a Gram-negative facultative intracellular bacterium and one of the most infectious pathogenic bacteria known. The bacterium is classified by the CDC as a Category A agent. The route of infection and the subtype of the bacterium influence the severity of the disease.
The most severe form of tularemia is caused by F. tularensis subsp. tularensis (type A), which occurs in North America, while F. tularensis subsp. holarctica (type B) is responsible for tularemia throughout the northern hemisphere. F. tularensis subsp. mediaasiatica is isolated in focal regions of Central Asia and has not been linked to human disease. F. tularensis subsp. novicida is rarely linked to human disease in North America and Australia. The second species of the genus Francisella, F. philomiragia, is an opportunistic pathogen that primarily causes serious disease in immunocompromised patients.
Humans become infected with Francisella tularensis bacteria through arthropod bites (ticks and flies), contact with infected tissues, consumption of contaminated water or food, and inhalation of infectious aerosols. The clinical picture of tularemia varies depending on the route of infection. While the most common symptom is ulceroglandular tularemia, pulmonary tularemia is the most severe form of the disease. The bacterium F. tularensis is considered a biological weapon because of its extreme infectivity, as 10 organisms can cause severe illness.
