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The Fusarium oxysporum (qPCR) product is used to detect sequences specific to pathogenic strains of Fusarium oxysporum in DNA preparations obtained from biological material collected from flax.
Kit size: 100 reactions
Reaction: duplex (FAM: Fusarium oxysporum, HEX: internal control)
Internal control: exogenous/endogenous
Determination: qualitative/quantitative
Kit components:
Fusarium oxysporum is a major pathogen of many important crops worldwide, causing crown and root rot as well as vascular wilt. The soil-borne fungus attacks a wide range of host plants, including onions, leeks, lettuce, tomatoes, brassicas, asparagus, cucurbits, peppers, coriander, spinach, basil, beans, peas, strawberries, watermelons, and bananas. It is also an important pathogen of non-food crops such as carnations and daffodils. Plant diseases caused by fungi of the genus Fusarium are collectively referred to as fusarium wilt.
Its wide range of hosts, as well as its economic and scientific impact, have led to F. oxysporum being recognized as the fifth most important plant pathogenic fungus. Fusarium oxysporum is a collection of subspecies/strains that includes both non-pathogenic and pathogenic isolates. Non-pathogenic isolates of F. oxysporum are commonly found in soil as saprophytes. Pathogenic isolates of F. oxysporum are distinguished as forms exhibiting host specificity (f. spp., formae spp.). To date, more than 120 forms of F. oxysporum with varying host specificity have been identified.
F. oxysporum secretes small effector proteins in the xylem, referred to as Secreted in Xylem (SIX), which contribute to its virulence. Most of these proteins contain cysteine residues in even numbers. These proteins are encoded by SIX genes located on mobile pathogenicity chromosomes. These are chromosomes that confer pathogenicity and undergo horizontal transfer.
To date, 14 SIX proteins have been described. The acquisition of mobile pathogenicity chromosomes encoding SIX proteins by non-pathogenic strains as a result of horizontal transfer results in the formation of new virulent strains. Some SIX genes present on pathogenicity chromosomes also contribute to defining host specificity variation among F. oxysporum strains.
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