Description
The Mycoplasma VET (qPCR) product is used to detect sequences specific to mycoplasmas associated with the urogenital tract in DNA preparations obtained from material collected from dogs.
Product characteristics
Kit size: 100 reactions
Reaction: duplex (FAM: Metamycoplasmataceae mycoplasmas , HEX: internal control)
Internal control: exogenous/endogenous
Determination: qualitative/quantitative
Kit components:
- Reaction mixture: contains DNA polymerase, probes and primers, and other qPCR reaction components;
- Positive control;
- Negative control;
- PCR-grade water;
- Internal control
Description of pathogens detected by Mycoplasma VET qPCR
To date, several species of mycoplasma have been linked to reproductive problems in dogs. The exact role of some species in reproductive disorders remains unclear because they are often isolated from both healthy and sick individuals. The most important species of mycoplasma include:
- Mycoplasmopsis arginini (Mycoplasma arginini)
- Mycoplasmopsis bovigenitalium (Mycoplasma bovigenitalium)
- Mesomycoplasma molare (Mycoplasma molare)
- Mycoplasmopsis edwardii (Mycoplasma edwardii
- Mycoplasmopsis cynos (Mycoplasma cynos)
- Metamycoplasma gateae
- Mycoplasmopsis felis (Mycoplasma felis)
- Mycoplasmopsis canis (Mycoplasma canis)
- Metamycoplasma spumans (Mycoplasma spumans)
- Mycoplasmopsis maculosa (Mycoplasma maculosum)
- Mycoplasmopsis opalescens (Mycoplasma opalescens)
Bacteria of the genus Mycoplasma (genus now called Mycoplasmopsis, class Mollicutes, order Mycoplasmatales, family Mycoplasmataceae) have been isolated from the reproductive tracts of both healthy and infertile dogs and bitches and are considered part of the normal vaginal flora.
The most common species of Mycoplasma found in the urogenital tract is Mycoplasma canis (now taxonomically named Mycoplasmopsis canis), identified in healthy and infertile dogs, including those with urogenital diseases, while M. edwardii (now taxonomically named Mycoplasmopsis edwardii) is most commonly found in healthy dogs. Other species identified in the canine reproductive tract include M. molare (now Mesomycoplasma molare), M. spumans (now Metamycoplasma spumans), and M. maculosum (now Mycoplasmopsis maculosa), which have variable but generally lower prevalence than M. canis.
Experimental studies have demonstrated the potential role of M. canis as a pathogen, and other Mycoplasma spp. have been linked to infertility in dogs or pyometra. Experimental and observational studies have established that mycoplasmas can cause endometritis, miscarriages, and neonatal mortality in many different species, such as cats, cattle, sheep, goats, and humans.



