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The Anaplasma/Ehrlichia/Borrelia/Babesia (qPCR) product is used to detect sequences specific to pathogens causing tick-borne diseases in DNA preparations obtained from ticks and material collected from animals.
Kit size: 100 markers
Reaction: fourplex (FAM: Lyme Disease group Borrelia, HEX: kinternal control, Texas Red: Anaplasma/Ehrlichia, Cy5: Babesia)
Internal control: exogenous/endogenous
Determination: qualitative/quantitative
Kit components:
Babesia are intracellular parasites that cause babesiosis, a disease similar to malaria. These pathogens attack the erythrocytes of mammals, including humans and dogs, leading to fever, anemia, jaundice, and organ complications, among other things. In Europe, the most common species threatening humans is Babesia divergens, although the presence of the less pathogenic B. microti has also been observed. In dogs, infections caused by B. canis, B. vogeli, and B. rossi are most commonly diagnosed. The disease is transmitted by ticks, and the incubation period can last up to several months. Early detection and implementation of antiprotozoal treatment is crucial for effective therapy.
Bacteria of the genera Anaplasma and Ehrlichia are intracellular pathogens transmitted by ticks, responsible for anaplasmosis and ehrlichiosis in humans and animals. These diseases most often manifest themselves as fever, muscle pain and headache, leukopenia, and thrombocytopenia. In humans, infections are mainly caused by Anaplasma phagocytophilum (anaplasmosis) and Ehrlichia chaffeensis (monocytic ehrlichiosis). Dogs can become ill as a result of infection with E. canis or A. platys, leading to symptoms such as fever, apathy, arthritis, and bleeding. Symptoms usually develop within a few days after the tick bite. Molecular diagnostics enable rapid detection of infection and initiation of antibiotic treatment.
Spirochetes of the genus Borrelia, responsible for Lyme disease, are the most commonly diagnosed tick-borne pathogens in the northern hemisphere. In Europe, the main pathogenic species are B. afzelii, B. garinii, and B. bavariensis, transmitted by the Ixodes ricinus tick. An early symptom of the disease is erythema migrans, but in later stages, joint pain, neurological symptoms, and even cognitive impairment may occur. Late-stage Lyme disease can occur months or years after infection, often leading to chronic symptoms. The symptoms are nonspecific, which is why rapid and accurate diagnosis is crucial. The use of molecular tests increases the chance of early detection and effective treatment of Lyme disease.
The Anaplasma/Ehrlichia/Borrelia/Babesia (qPCR) product allows for the detection of three groups of pathogens causing tick-borne diseases:
The pathogens above can be detected qualitatively. They are detected in a fourplex reaction. Bacteria of the genera Anaplasma and Ehrlichia are determinated.
