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The Toxoplasma gondii (qPCR) product is used to detect sequences specific to the protozoan Toxoplasma gondii in DNA preparations obtained from animal feces, food, water samples, and animal tissues.
Kit size: 100 reactions
Reaction: duplex (FAM: Toxoplasma gondii, HEX: internal control)
Internal control: exogenous/endogenous
Determination: qualitative/quantitative
Kit components:
Toxoplasma gondii is a cosmopolitan species. It occurs in approximately 200 species of birds and mammals, but its definitive host is felines, in whose digestive tract the parasite reproduces sexually, forming oocysts. Oocysts are excreted in feces and accidentally eaten by other animals, including rodents. The parasite then forms cysts in various organs, including the nervous system. The parasite’s life cycle is completed when an infected animal (rat or mouse) is eaten by a cat.
Infection with T. gondii causes a disease in animals known as toxoplasmosis. In humans, this protozoan causes a zoonotic disease (zoonosis). Infection occurs directly, e.g., by eating dirty vegetables containing cysts, or indirectly by eating undercooked meat. It is also possible for the infection to be transmitted from a woman to her fetus through the placenta, or as a result of contact with the feces of a cat that is a carrier.
Toxoplasma gondii is an important opportunistic infectious pathogen affecting organ transplant recipients, AIDS patients, and other immunocompromised patients. Toxoplasmic encephalitis and extracerebral toxoplasmosis are among the major life-threatening infections of T. gondii in these patients. In addition, toxoplasmic infection during pregnancy can lead to severe, if not fatal, infection. If the fetus is infected during the first trimester, the result is spontaneous abortion, stillbirth, or severe illness. If infection occurs after the first trimester, symptoms of the disease include epilepsy, encephalitis, retardation, blindness, and other neurological disorders.
