Description
The Tritrichomonas foetus (qPCR) product is used to detect sequences specific to the protozoan Tritrichomonas foetus in DNA preparations obtained from material collected from cattle and cats.
Product characteristics
Kit size: 100 reactions
Reaction: duplex (FAM: Tritrichomonas foetus, HEX: internal control)
Internal control: exogenous/endogenous
Determination: qualitative/quantitative
Kit components:
- Reaction mixture: contains DNA polymerase, probes and primers, and other qPCR reaction components;
- Positive control;
- Negative control;
- PCR-grade water;
- Internal control
Description of the protozoan Tritrichomonas foetus
The protozoan Tritrichomonas foetus is a pathogen of the reproductive system of cattle and the digestive tract of cats. In cows, it causes a disease called trichomoniasis. In cats, Tritrichomonas fetus causes recurrent diarrhea. T. fetus looks similar to Giardia and is often misdiagnosed under a microscope.
Trichomoniasis in cattle is a sexually transmitted disease found worldwide. The disease is prevalent in areas where natural mating is used, such as Asia, Australia, South America, South Africa, Spain, and the United States. Infection of the female vagina and uterus occurs through contact with the bull’s prepuce during mating. Bulls can become infected at any age, but those aged 3 years and older are more susceptible to infection, becoming asymptomatic carriers that transmit the parasites to cows during sexual intercourse.
Transmission of the pathogen from bull to cow is very efficient; a single mating with an infected bull will result in infection in 95% of susceptible heifers. Cows can transmit the infection after pregnancy and potentially serve as a reservoir of infection for virgin or uninfected bulls. Typical symptoms of trichomoniasis in cows include cervicitis, endometritis, first-trimester fetal death, delayed return to estrus, and infertility.
In cats, Tritrichomonas fetus infection is characterized by intermittent diarrhea, which may sometimes contain blood and mucus. The diarrhea is partially formed and has the consistency of cow patties. In most cases, it affects cats 12 months of age or younger and cats from shelters and homes with multiple cats. The pathogen is transmitted through close and direct contact between animals. There is no evidence that T. fetus in cats is sexually transmitted or infects the reproductive system or mammary glands of cats.



