We specialize in nucleic acid sequencing using Real Time PCR (qPCR) technology. We have experience in using qPCR technology for both qualitative and quantitative determinations.
We specialize in nucleic acid sequencing using Real Time PCR (qPCR) technology. We have experience in using qPCR technology for both qualitative and quantitative determinations.
Nucleic acid extraction is a highly important process designed to isolate nucleic acids from the starting biological material. Properly performed extraction allows for the production of high-quality nucleic acid preparations free of contaminants (e.g., PCR inhibitors), which is essential for obtaining reliable analysis results. Another important thing is high extraction yield, especially when the amount of starting material is small.
Real Time PCR Technology (qPCR) is currently a fundamental tool used for quantitative and qualitative determination of specific DNA/RNA sequences in tested preparations. This is possible thanks to several important features of this technique, such as high precision of determination, high specificity of the reaction, and the quantitative nature of the technique in a wide range of analyte concentrations.
Genotyping is the process of determining the genotype of a given individual by identifying its DNA sequence. Most modern genotyping techniques are based on the Real Time PCR method (e.g., HRM, Taqman probe genotyping, AS-qPCR, etc.). Their advantages include short analysis time, relatively low cost, and high reliability of the results obtained.
Gene expression analysis is one example of the quantitative determination of nucleic acids. It involves measuring quantitative changes in the levels of specific transcripts occurring in a cell in response to both internal and external factors. Tracking quantitative changes in the expression levels of specific gene groups allows for the monitoring of processes occurring in the cell and the identification of mechanisms through which a given factor influences cell function.
Real Time PCR Technology (qPCR) is currently a fundamental tool used for quantitative and qualitative determination of specific DNA/RNA sequences in tested preparations. This is possible thanks to several important features of this technique, such as high precision of determination, high specificity of the reaction, and the quantitative nature of the technique in a wide range of analyte concentrations.
Nucleic acid extraction is a highly important process designed to isolate nucleic acids from the starting biological material. Properly performed extraction allows for the production of high-quality nucleic acid preparations free of contaminants (e.g., PCR inhibitors), which is essential for obtaining reliable analysis results. Another important thing is high extraction yield, especially when the amount of starting material is small.
Genotyping is the process of determining the genotype of a given individual by identifying its DNA sequence. Most modern genotyping techniques are based on the Real Time PCR method (e.g., HRM, Taqman probe genotyping, AS-qPCR, etc.). Their advantages include short analysis time, relatively low cost, and high reliability of the results obtained.
Gene expression analysis is one example of the quantitative determination of nucleic acids. It involves measuring quantitative changes in the levels of specific transcripts occurring in a cell in response to both internal and external factors. Tracking quantitative changes in the expression levels of specific gene groups allows for the monitoring of processes occurring in the cell and the identification of mechanisms through which a given factor influences cell function.
We help implement scientific projects and research and development projects related to nucleic acid analysis. Our experience allows us to provide support in the proper planning of experiments, their implementation, and the analysis of their results, including statistical analysis. We own equipment that allows us to perform some of the experiments related to the extraction and analysis of nucleic acids in our laboratory.
We develop research and diagnostic methods based on nucleic acid analysis. The service includes the selection and optimization of nucleic acid extraction methods and nucleic acid analysis methods. We assess the quality and scope of application of the developed method by validating it within an agreed scope. We specialize in methods based on the qPCR technology, but it is also possible to use other technologies (e.g., PCR, sequencing, etc.).
We perform analyses related to the determination of nucleic acid sequences on request. The starting material for the determination can be appropriately secured biological material or a prepared DNA/RNA sample. We perform both quantitative and qualitative determinations. The determination can be performed according to the protocol provided or according to a method developed in our laboratory.
For the institutions that would like to improve qualifications of their employees, we offer the opportunity to conduct closed training courses on qPCR (Real Time PCR) technology and related areas (e.g., nucleic acid extraction from specific material) in their own laboratories.
We offer institutions that would like to improve the qualifications of their employees the opportunity to conduct closed training courses on qPCR (Real Time PCR) technology and related areas (e.g., nucleic acid extraction from specific material) in their own laboratories.
Our laboratory is located in the Wrocław Technology Park. Its equipment is optimized for working with nucleic acids. In addition to the well-equipped laboratory, we have access to software that allows us to design PCR/qPCR reactions for various applications. In our work, we use high-quality reagents and materials with appropriate certificates. We ensure that our suppliers have quality control systems in place. This allows us to provide high-quality services.
Thanks to our location within the Wrocław Technology Park, we have access to modern laboratories and professional equipment (including PCR chambers, laminar chambers, spectrophotometers, PCR/qPCR devices, gel electrophoresis and gel documentation devices, refrigerators, including a cold room, freezers, low-temperature freezers, water demineralization devices, etc.). In addition, over 200 companies are associated with the WPT, including companies in the field of biotechnology. Cooperation with them further expands our capabilities and the scope of projects we can implement.
We specialize in quantitative and qualitative determination of nucleic acids using qPCR technology and DNA sequence genotyping using various techniques. We have many years of experience in developing research and diagnostic methods based on DNA sequence analysis using PCR/qPCR technology. We design qPCR reactions for various applications, such as the diagnosis of infectious and genetic diseases and detection and quantitative determination of microorganisms in various materials (clinical material, food, environmental samples, etc.).
The laboratory is located in the Wrocław Technology Park. Its equipment is optimized for working with nucleic acids. In addition to the well-equipped laboratory, we have access to software that allows us to design PCR/qPCR reactions for various applications. In our work, we use high-quality reagents and materials with appropriate certificates. We ensure that our suppliers have quality control systems in place. This allows us to provide high-quality services.
Thanks to our location within the Wrocław Technology Park, we have access to modern laboratories and professional equipment (including PCR chambers, laminar chambers, spectrophotometers, PCR/qPCR devices, gel electrophoresis and gel documentation devices, refrigerators, including a cold room, freezers, low-temperature freezers, water demineralization devices, etc.). In addition, over 200 companies are associated with the WPT, including companies in the field of biotechnology. Cooperation with them further expands our capabilities and the scope of projects we can implement.
We specialize in quantitative and qualitative determination of nucleic acids using qPCR technology and DNA sequence genotyping using various techniques. We have many years of experience in developing research and diagnostic methods based on DNA sequence analysis using PCR/qPCR technology. We design qPCR reactions for various applications, such as the diagnosis of infectious and genetic diseases and detection and quantitative determination of microorganisms in various materials (clinical material, food, environmental samples, etc.).